2JCA
Crystal structure of the streptomyces coelicolor holo- [Acyl-carrier-protein] Synthase (AcpS) at 2 A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-23 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.940, 75.940, 108.351 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.800 - 1.980 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f7t |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.690 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.080 | 0.380 |
| Number of reflections | 25759 | |
| <I/σ(I)> | 22.19 | 2.04 |
| Completeness [%] | 99.3 | 95.7 |
| Redundancy | 6.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.8 | ACPS WAS DIALYZED IN 50 MM TRIS PH 8.8, 10% GLYCEROL. THEN IT WAS CRYSTALLIZED IN 0.2M AMMONIUM SULPHATE, 0.1M NACACODYLATE PH 6.5, 30% PEG8000 |
