2JC6
Crystal structure of human calmodulin-dependent protein kinase 1D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.640, 93.060, 101.860 |
| Unit cell angles | 90.00, 96.16, 90.00 |
Refinement procedure
| Resolution | 101.020 - 2.300 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a06 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.428 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.200 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.440 |
| Number of reflections | 29986 | |
| <I/σ(I)> | 11.17 | 2.56 |
| Completeness [%] | 91.6 | 99.9 |
| Redundancy | 3.47 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M CITRATE PH 5.6, 20% ISOPROPANOL, 20% PEG 4K. |
