2JBR
Structure of the monooxygenase component of p-hydroxyphenylacetate hydroxylase from Acinetobacter baumanni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 91.441, 182.077, 287.348 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.070 - 2.300 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.23500 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.083 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.480 |
| Number of reflections | 105406 | |
| <I/σ(I)> | 12.8 | 1.9 |
| Completeness [%] | 99.1 | 94.8 |
| Redundancy | 4.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | pH 6.80 |
