Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 263 |
Detector technology | IMAGE PLATE |
Collection date | 1992-11-13 |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 223.500, 223.500, 113.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.560 - 2.400 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4PRC |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.560 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.090 | 0.310 |
Number of reflections | 103499 | |
<I/σ(I)> | 6.9 | 1.6 |
Completeness [%] | 92.3 | 86.2 |
Redundancy | 1.9 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | pH 6.00 |