2JBA
PhoB response regulator receiver domain constitutively-active double mutant D53A and Y102C.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.907, 47.631, 59.703 |
| Unit cell angles | 90.00, 99.34, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.450 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b00 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.309 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.700 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.070 | 0.290 |
| Number of reflections | 41827 | |
| <I/σ(I)> | 15.2 | 2.3 |
| Completeness [%] | 94.5 | 70.3 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 3 MICROLITER OF PROTEIN SOLUTION AT 5.5 MG/ML AND 3 MICROLITER OF RESERVOIR SOLUTION (20% (W/V) PEG 4K, 0.4M SODIUM ACETATE, 0.1M TRISHCL (PH 8), 0.01M DTT |
