2JB9
PhoB response regulator receiver domain constitutively-active double mutant D10A and D53E.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.924, 60.157, 116.492 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.224 |
| Rwork | 0.223 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b00 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.940 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.900 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.350 |
| Number of reflections | 24245 | |
| <I/σ(I)> | 14.3 | 4 |
| Completeness [%] | 93.0 | 96.6 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 3 MICROLITER OF PROTEIN SOLUTION AT 5.6 MG/ML AND 6 MICROLITER OF RESERVOIR SOLUTION (22.5% PEG 4K, 0.4 M SODIUM ACETATE, 0.1 M TRISHCL, PH 8) |
