2JB2
The structure of L-amino acid oxidase from Rhodococcus opacus in complex with L-phenylalanine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.053, 110.059, 134.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.990 - 1.450 |
| R-factor | 0.173 |
| Rwork | 0.172 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jae |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.120 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.100 | 0.480 |
| Number of reflections | 157853 | |
| <I/σ(I)> | 11.9 | 2.1 |
| Completeness [%] | 91.3 | 86.1 |
| Redundancy | 3.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 100MM HEPES PH 7.8 10% 2-PROPANOLE 10% PEG 4000 |
