2JAX
Universal Stress Protein Rv2623 from Mycobaterium Tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 102.134, 102.134, 158.068 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 88.390 - 3.220 |
| R-factor | 0.27 |
| Rwork | 0.268 |
| R-free | 0.32400 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.792 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.320 |
| High resolution limit [Å] | 3.220 | 3.220 |
| Rmerge | 0.130 | 0.340 |
| Number of reflections | 8342 | |
| <I/σ(I)> | 23 | 6 |
| Completeness [%] | 99.2 | 98.7 |
| Redundancy | 11.6 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.2M CACL2 0.1M NAACT PH4.6 20% ISOPROPANOL |
