2JAM
Crystal structure of human calmodulin-dependent protein kinase I G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-19 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.666, 53.736, 64.177 |
| Unit cell angles | 65.45, 71.91, 73.84 |
Refinement procedure
| Resolution | 56.890 - 1.700 |
| R-factor | 0.157 |
| Rwork | 0.155 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a06 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.364 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.360 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.280 |
| Number of reflections | 64017 | |
| <I/σ(I)> | 12.82 | 2.97 |
| Completeness [%] | 98.9 | 93 |
| Redundancy | 2.79 | 2.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.20M NA(MALONATE), 0.1M BTPROP PH 7.5, 20.0% PEG 3350, 10.0% ETGLY |
