2JAK
Human PP2A regulatory subunit B56g
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 105.800, 105.800, 82.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.470 - 2.600 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.26000 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.383 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE/RESOLVE |
| Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.050 | 0.560 |
| Number of reflections | 16638 | |
| <I/σ(I)> | 30.11 | 4.7 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 13.9 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 29% MPD 0.1M TRIS, PH 8.0 20MM L-PROLINE |
