2JAE
The structure of L-amino acid oxidase from Rhodococcus opacus in the unbound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.696, 109.705, 134.402 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.980 - 1.250 |
| R-factor | 0.154 |
| Rwork | 0.152 |
| R-free | 0.18200 |
| Structure solution method | SAD |
| RMSD bond length | 0.060 |
| RMSD bond angle | 1.082 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.220 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.080 | 0.600 |
| Number of reflections | 290588 | |
| <I/σ(I)> | 34.4 | 3.9 |
| Completeness [%] | 99.2 | 92.5 |
| Redundancy | 13.4 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 100MM HEPES PH 7.8 10% 2-PROPANOL 10% PEG4000 |
