2JAD
Yellow fluorescent protein - glutaredoxin fusion protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 64 |
| Unit cell lengths | 132.111, 132.111, 58.722 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h6r |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.360 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.100 | 0.360 |
| Number of reflections | 15714 | |
| <I/σ(I)> | 5.8 | 2.1 |
| Completeness [%] | 96.7 | 95.1 |
| Redundancy | 2.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10 | 50MM BICARBONATE PH 10 1.5-1.75M MGSO4 |
