2JAB
A designed ankyrin repeat protein evolved to picomolar affinity to Her2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-12-10 |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 152.080, 52.191, 70.164 |
Unit cell angles | 90.00, 105.19, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.700 |
R-factor | 0.18 |
Rwork | 0.178 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1MJ0 AND 2BKG |
RMSD bond length | 0.014 |
RMSD bond angle | 1.285 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.300 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.120 | 0.880 |
Number of reflections | 58105 | |
<I/σ(I)> | 13.2 | 1.3 |
Completeness [%] | 98.6 | 93.3 |
Redundancy | 3.8 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | PROTEIN: 6 MG/ML IN 20 MM SODIUM PHOSPHATE, 75 MM NACL PH 7.4. PRECIPITANT: 0.1 M TRISHCL (PH 8.5), 2.3 M (NH4)2SO4, 10% GLYCEROL (V/V). ROOM TEMPERATURE. |