2JA9

Structure of the N-terminal deletion of yeast exosome component Rrp40

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	I 41 3 2
Unit cell lengths	153.510, 153.510, 153.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.910 - 2.200
R-factor	0.19
Rwork	0.189
R-free	0.20600
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.997
Phasing software	SHELX
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200	2.200
Rmerge	0.120	0.590
Number of reflections	15115
<I/σ(I)>	34	8.9
Completeness [%]	99.0	97.2
Redundancy	45	36

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				20% (W/V) PEG 3350,200 MM NA2SO4