2JA8
CPD lesion containing RNA Polymerase II elongation complex D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-14 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 222.642, 392.845, 282.894 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.800 |
R-factor | 0.2686 |
Rwork | 0.269 |
R-free | 0.28760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y1w |
RMSD bond length | 0.009 |
RMSD bond angle | 1.550 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.940 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.080 | 0.370 |
Number of reflections | 121341 | |
<I/σ(I)> | 17.1 | 3.2 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 5.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7.0 |