2JA6
CPD lesion containing RNA Polymerase II elongation complex B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-22 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 223.580, 393.491, 283.505 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 4.000 |
| R-factor | 0.2923 |
| Rwork | 0.292 |
| R-free | 0.30090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y1w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.520 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.140 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.090 | 0.410 |
| Number of reflections | 104987 | |
| <I/σ(I)> | 15.8 | 3.1 |
| Completeness [%] | 99.8 | 98.2 |
| Redundancy | 5.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.0 |
