2JA4
Crystal structure of CD5 domain III reveals the fold of a group B scavenger cysteine-rich receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 62.931, 62.931, 72.564 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.180 - 2.210 |
| R-factor | 0.196 |
| Rwork | 0.193 |
| R-free | 0.24800 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.156 |
| Data scaling software | SCALEPACK |
| Phasing software | BNP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.050 | 0.240 |
| Number of reflections | 12098 | |
| <I/σ(I)> | 15.3 | 2.2 |
| Completeness [%] | 96.9 | 95.6 |
| Redundancy | 5.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 15-25% PEG 8000, 100 MM NAAC PH 4.4, 200 MM LI2SO4 |
