2J9Z

Tryptophan Synthase T110 mutant complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Spacegroup name	C 1 2 1
Unit cell lengths	182.800, 59.900, 67.600
Unit cell angles	90.00, 94.40, 90.00

Refinement procedure

Resolution	19.850 - 1.800
R-factor	0.187
Rwork	0.187
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.120
RMSD bond angle	1.700
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.120	0.330
Number of reflections	64747
<I/σ(I)>	7.1	2
Completeness [%]	95.5	90.2
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.8		pH 7.8