2J9W
Structural insight into the ESCRT-I-II link and its role in MVB trafficking
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-12 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 25.997, 89.713, 40.384 |
Unit cell angles | 90.00, 95.13, 90.00 |
Refinement procedure
Resolution | 44.860 - 1.300 |
R-factor | 0.202 |
Rwork | 0.200 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SE DATASET FROM SEMET SUBSTITUTED CRYSTAL |
RMSD bond length | 0.014 |
RMSD bond angle | 1.502 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.860 | 2.230 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.100 | 0.330 |
Number of reflections | 8573 | |
<I/σ(I)> | 10.57 | 3.28 |
Completeness [%] | 88.3 | 45.7 |
Redundancy | 4.3 | 1.17 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 15-25% PEG 600, 0.15M POTASSIUM THIOCYANATE, 0.2M SODIUM CHLORIDE, 0.1M GUANIDINE HYDROCHLORIDE, 0.1M SODIUM CACODYLATE PH 6.5 |