2J9V
2 Angstrom X-ray structure of the yeast ESCRT-I Vps28 C-terminus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-12 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 44.690, 128.581, 50.276 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.220 - 2.000 |
R-factor | 0.229 |
Rwork | 0.228 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SE DATASET FROM SEMET SUBSTITUTED CRYSTAL OF DIFFERENT CRYSTAL FORM |
RMSD bond length | 0.017 |
RMSD bond angle | 1.718 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.280 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.100 | 0.410 |
Number of reflections | 10123 | |
<I/σ(I)> | 5.52 | 1.81 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 6.09 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 4% PEG 400, 0.1M LITHIUM SULPHATE, 0.1M SODIUM CITRATE (PH5.6), pH 5.60 |