2J9G
Crystal structure of Biotin carboxylase from E. coli in complex with AMPPNP and ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Collection date | 2007-05-04 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.704, 112.251, 121.769 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 82.480 - 2.050 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dv2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.064 |
| Data reduction software | SCALEPACK |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.080 | 0.560 |
| Number of reflections | 59125 | |
| <I/σ(I)> | 29.97 | 4.22 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M BIS-TRIS PH 6.5, 0.2M CACL2, 45% MPD |
