2J98
Human coronavirus 229E non structural protein 9 cys69ala mutant (Nsp9)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-18 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 26.396, 61.377, 107.309 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.680 - 1.800 |
R-factor | 0.218 |
Rwork | 0.214 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qz8 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.639 |
Data reduction software | HKL (SUITE) |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.800 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 0.080 | 0.500 |
Number of reflections | 17889 | |
<I/σ(I)> | 26.9 | 3.07 |
Completeness [%] | 94.0 | 44.6 |
Redundancy | 8 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | 0.1M NA ACETATE PH 4.6 30% PEG MME 2000 |