2J97
Human coronavirus 229E non structural protein 9 (Nsp9)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 85.629, 85.629, 48.689 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 74.120 - 1.750 |
| R-factor | 0.192 |
| Rwork | 0.191 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qz8 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.417 |
| Data reduction software | HKLSUITE |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.620 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.110 | 0.820 |
| Number of reflections | 14777 | |
| <I/σ(I)> | 11.4 | 2.6 |
| Completeness [%] | 98.7 | 73.4 |
| Redundancy | 11.3 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 1.9M AMMONIUM SULFATE 0.1M NA ACETATE PH 4.0 5% MPD |
