2J96
The E-configuration of alfa-Phycoerythrocyanin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-10-01 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.808, 102.598, 32.146 |
| Unit cell angles | 90.00, 94.48, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.250 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.30860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c7l |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.683 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BEAST |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.090 | 0.030 |
| Number of reflections | 53396 | |
| <I/σ(I)> | 7.6 | 2.3 |
| Completeness [%] | 93.3 | 93.3 |
| Redundancy | 2.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 291 | 1.2 M KPI PH 7.0,0.2 M MGSO4 AT 18 DEG C ILLUMINATION BY 587 NM CRYSTALLIZATION IN THE DARK |
