2J8Z
Crystal Structure of human P53 inducible oxidoreductase (TP53I3,PIG3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 46.860, 46.860, 315.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iyz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.302 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.380 |
| Number of reflections | 13313 | |
| <I/σ(I)> | 12.1 | 4.4 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 6.58 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 0.22M LITHIUM SULPHATE, 0.1M SODIUM ACETATE PH4.0, 45% PEG300, pH 4.00 |
