2J8W
The crystal structure of cytochrome c' from Rubrivivax gelatinosus at 1.3 A Resolution and pH 8.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-10-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 69.630, 69.630, 123.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.960 - 1.290 |
| R-factor | 0.151 |
| Rwork | 0.151 |
| R-free | 0.17900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jaf |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.744 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.940 | 1.310 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Rmerge | 0.040 | 0.380 |
| Number of reflections | 87744 | |
| <I/σ(I)> | 33.7 | 3.58 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 5.1 | 3.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | HANGING DROP 2.5 M AMMONIUM SULPHATE 0.1M TRIS-HCL PH8.0 |
