2J8O
Structure of the immunoglobulin tandem repeat of titin A168-A169
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-03 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 95.560, 122.487, 98.699 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.000 - 2.490 |
R-factor | 0.252 |
Rwork | 0.249 |
R-free | 0.30000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j8h |
RMSD bond length | 0.015 |
RMSD bond angle | 1.648 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.570 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.040 | 0.460 |
Number of reflections | 20714 | |
<I/σ(I)> | 43.1 | 5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | AMMONIUM SULFATE, BICINE, pH 9 |