2J8K
Structure of the fusion of NP275 and NP276, pentapeptide repeat proteins from Nostoc punctiforme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 194 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.605, 55.470, 58.989 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.030 - 1.500 |
| R-factor | 0.175 |
| Rwork | 0.174 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j8i |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.412 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.900 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.030 | 0.120 |
| Number of reflections | 26666 | |
| <I/σ(I)> | 31.8 | 11.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.6 M AMMONIUM SULFATE 100 MM MES PH 6.5 PROTEIN 10 MG/ML |
