2J8C
X-ray high resolution structure of the photosynthetic reaction center from Rb. sphaeroides at pH 8 in the neutral state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-05 |
| Detector | MARRESERACH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 138.690, 138.690, 184.613 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 119.520 - 1.870 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pcr |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.438 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 199.520 | 1.890 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.040 | 0.200 |
| Number of reflections | 144184 | |
| <I/σ(I)> | 23.7 | 2.83 |
| Completeness [%] | 87.0 | 70.3 |
| Redundancy | 1.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8 |
