2J8B
High resolution structure of human CD59
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-21 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.92, 1.24 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.948, 45.918, 46.033 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.150 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cdq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.410 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.200 | 1.160 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.100 | 0.240 |
| Number of reflections | 27724 | |
| <I/σ(I)> | 20.7 | 3.7 |
| Completeness [%] | 99.0 | 93.3 |
| Redundancy | 12 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 28 % PEG 4000 0.25M AMMONIUM SULPHATE |
