2J88

Hyaluronidase in complex with a monoclonal IgG Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-24
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	151.563, 70.050, 138.880
Unit cell angles	90.00, 123.68, 90.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.212
Rwork	0.210
R-free	0.24700
Structure solution method	MAD
Starting model (for MR)	PDB ENTRIES 1FCQ 1a7q 15c8
RMSD bond length	0.009
RMSD bond angle	1.136
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.630	2.740
High resolution limit [Å]	2.500	2.600
Rmerge	0.080	0.300
Number of reflections	32047
<I/σ(I)>	6.98	2.15
Completeness [%]	85.4	81.8
Redundancy	3.17	3.19

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9.5		10% PEG 8000, 0.2M NACL, 0.1M CHES PH9.5, pH 9.50