2J86
Structural analysis of the PP2C Family Phosphatase tPphA of Thermosynechococcus elongatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 89.5 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 113.290, 113.290, 88.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.050 |
| R-factor | 0.2402 |
| Rwork | 0.240 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j82 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.303 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.150 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.040 | 0.160 |
| Number of reflections | 11417 | |
| <I/σ(I)> | 15.72 | 5.22 |
| Completeness [%] | 99.9 | 98.7 |
| Redundancy | 1.86 | 1.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS PH 7.0, 25 % PEG 3350 |
