2J7X
STRUCTURE OF ESTRADIOL-BOUND ESTROGEN RECEPTOR BETA LBD IN COMPLEX WITH LXXLL MOTIF FROM NCOA5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 62.550, 62.550, 171.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.100 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qkn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.391 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.060 | 0.390 |
| Number of reflections | 20776 | |
| <I/σ(I)> | 11.7 | 5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 15 | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 5-7% PEG 8K, 5-7% PEG 1K, 0.13M LITHIUM SULPHATE, 0.1M TRIS PH 8.0 . |
