2J7O

STRUCTURE OF THE RNAI POLYMERASE FROM NEUROSPORA CRASSA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	114.596, 125.015, 102.204
Unit cell angles	90.00, 108.75, 90.00

Refinement procedure

Resolution	20.000 - 3.500
R-factor	0.3531
Rwork	0.353
R-free	0.35970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.630
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.600
High resolution limit [Å]	3.450	3.450
Rmerge	0.210
Number of reflections	22247
<I/σ(I)>	8.7	2.4
Completeness [%]	100.0	100
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1