2J7N
Structure of the RNAi polymerase from Neurospora crassa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 101.021, 122.553, 114.701 |
Unit cell angles | 90.00, 108.90, 90.00 |
Refinement procedure
Resolution | 19.980 - 2.300 |
R-factor | 0.219 |
Rwork | 0.217 |
R-free | 0.26400 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.524 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.100 | |
Number of reflections | 114252 | |
<I/σ(I)> | 18.2 | 1.3 |
Completeness [%] | 97.9 | 100 |
Redundancy | 15.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 100 MM TRISHCL PH 7.5, 150 MM NACL, 8% PEG 6000, AND 5 MM MGCL2. |