2J7K
Crystal structure of the T84A mutant EF-G:GDPCP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-21 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.230, 88.680, 114.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.000 - 2.900 |
Rwork | 0.226 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bm0 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.070 | 0.470 |
Number of reflections | 17014 | |
<I/σ(I)> | 18.6 | 3.2 |
Completeness [%] | 95.4 | 98.7 |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.3 | 17 % PEG8000 100 MM HEPES 46 MM TRIS 10 MM MGCL2 10 MM GDPCP, pH 7.3 |