2J7J
Invariance of the zinc finger module: a comparison of the free structure with those in nucleic-acid complexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-24 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9791, 1.2824, 1.2828, 1.2651 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 50.954, 50.954, 173.431 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.280 - 1.650 |
| R-factor | 0.218 |
| Rwork | 0.217 |
| R-free | 0.22800 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.878 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.830 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.090 | 0.380 |
| Number of reflections | 17038 | |
| <I/σ(I)> | 16.2 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.5 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 1.6M (NH4)2SO4, 25MM MGSO4, 50MM TRIS.HCL PH8.5 |
