2J78
Beta-glucosidase from Thermotoga maritima in complex with gluco- hydroximolactam
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-21 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 96.950, 91.459, 111.527 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 72.550 - 1.650 |
R-factor | 0.166 |
Rwork | 0.164 |
R-free | 0.20100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1od0 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.395 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.920 | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.070 | 0.420 |
Number of reflections | 119424 | |
<I/σ(I)> | 44.3 | 4.3 |
Completeness [%] | 98.1 | 98.1 |
Redundancy | 5.2 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 10 MG/ML PROTEIN 15% PEG 4K 0.1 IMIDAZOLE, PH 7 0.2 M CALCIUM ACETATE 25% ETHYLENE GLYCOL AS CRYO |