2J73
alpha-glucan rcognition by a family 41 carbohydrate-binding module from Thermotoga maritima pullulanase PulA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.528, 37.037, 54.910 |
| Unit cell angles | 90.00, 112.01, 90.00 |
Refinement procedure
| Resolution | 50.640 - 1.400 |
| R-factor | 0.152 |
| Rwork | 0.149 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j71 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.856 |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.040 | 0.260 |
| Number of reflections | 35936 | |
| <I/σ(I)> | 17.8 | 2.8 |
| Completeness [%] | 91.2 | 68.4 |
| Redundancy | 3.65 | 2.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
