2J71
alpha-glucan recognition by a family 41 carbohydrate-binding module from Thermotoga maritima pullulanase PulA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 112.504, 112.504, 83.739 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.250 - 1.690 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.976 |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.810 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.040 | 0.380 |
| Number of reflections | 22721 | |
| <I/σ(I)> | 14.6 | 2.8 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 6.84 | 5.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
