2J6Y
Structural and Functional Characterisation of partner switching regulating the environmental stress response in Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-06 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.157, 47.831, 94.968 |
Unit cell angles | 90.00, 104.36, 90.00 |
Refinement procedure
Resolution | 42.910 - 1.850 |
R-factor | 0.186 |
Rwork | 0.183 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w53 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.757 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | ARP/wARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.110 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.060 | 0.440 |
Number of reflections | 35972 | |
<I/σ(I)> | 5.3 | 1.7 |
Completeness [%] | 96.1 | 93.1 |
Redundancy | 2.2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 10 % PEG 20000, 100 MM MES PH 6.5 |