2J6S
Ternary complex of Sulfolobus solfataricus Dpo4 DNA polymerase, O6- methylguanine modified DNA, and dATP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-22 |
Detector | BRUKER |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 93.682, 103.271, 52.674 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.090 - 2.500 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c22 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.100 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.100 | 0.670 |
Number of reflections | 18215 | |
<I/σ(I)> | 14.36 | 2.51 |
Completeness [%] | 99.5 | 100 |
Redundancy | 6.69 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | 7.5% PEG 3350, 25 MM TRIS_HCL PH 7.4, 100 MM CA(CH3CO2)2, 50MM NACL, 2.5% GLYCEROL, 5MM CACL2, AND 1MM DATP. |