2J6I
Candida boidinii formate dehydrogenase (FDH) C-terminal mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.360, 68.140, 109.150 |
| Unit cell angles | 78.07, 89.41, 81.27 |
Refinement procedure
| Resolution | 19.260 - 1.550 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nac |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.268 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.500 | 1.900 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.150 | 0.500 |
| Number of reflections | 196828 | |
| <I/σ(I)> | 12 | 3 |
| Completeness [%] | 95.9 | 94.2 |
| Redundancy | 2.4 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1 M SODIUM-CACODYLATE PH 6.0 0.2 M AMMONIUM-SULPHATE 32.5 % W/V PEG 8K |
