2J66

Structural characterisation of BtrK decarboxylase from butirosin biosynthesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	287
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	71.604, 128.814, 45.323
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.650
R-factor	0.2126
Rwork	0.213
R-free	0.22350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1K00
RMSD bond length	0.000
RMSD bond angle	0.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.690
High resolution limit [Å]	1.650	1.650
Rmerge	0.040	0.390
Number of reflections	51193
<I/σ(I)>	13	49.7
Completeness [%]	99.8	100
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1