2J5V
GLUTAMATE 5-KINASE FROM ESCHERICHIA COLI COMPLEXED WITH GLUTAMYL-5-PHOSPHATE AND PYROGLUTAMIC ACID
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-10 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 101.455, 101.455, 178.728 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 88.390 - 2.500 |
R-factor | 0.195 |
Rwork | 0.192 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ako |
RMSD bond length | 0.011 |
RMSD bond angle | 1.353 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.800 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.070 | 0.420 |
Number of reflections | 61566 | |
<I/σ(I)> | 6.8 | 1.7 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 11.1 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 1.6 M MGSO4, 0.1 M KCL, 0.1 M MES PH 6.5 |