2J5T
Glutamate 5-kinase from Escherichia coli complexed with glutamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-06 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 96.302, 124.108, 144.927 |
Unit cell angles | 90.00, 93.96, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.900 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PARTIALLY-REFINED MODEL OF PBD ENTRY 2J5V |
RMSD bond length | 0.009 |
RMSD bond angle | 1.159 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.160 | 0.490 |
Number of reflections | 74908 | |
<I/σ(I)> | 9.7 | 2.9 |
Completeness [%] | 99.3 | 99.6 |
Redundancy | 4.5 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 1.45 M MGSO4, 20 MM CACL2, 0.1 M MES PH 6.5. |