2J5B

Structure of the Tyrosyl tRNA synthetase from Acanthamoeba polyphaga Mimivirus complexed with tyrosynol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-19
Detector	ADSC QUANTUM 210
Spacegroup name	P 21 21 21
Unit cell lengths	63.502, 107.300, 148.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.830 - 2.200
R-factor	0.216
Rwork	0.216
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	LOW RESOLUTION MAD STRUCTURE
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.090	0.290
Number of reflections	52537
<I/σ(I)>	2.2	2.3
Completeness [%]	100.0	100
Redundancy	4.5	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		RESERVOIR: 0.1 M SODIUM CITRATE BUFFER PH 5.5, PEG 4000 8% (W/V), 15% MPD (V/V), 0.1M KCL, 1MM MGCL2 DROPLET: 2 MICROL TYRRS 14 MG/ML IN 20 MM TRIS PH 7.4, 1MM TYROSINOL, 1MM ATP 0.5 MICROL RESERVOIR