2J56
X-ray reduced Paraccocus denitrificans methylamine dehydrogenase N- semiquinone in complex with amicyanin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 123.029, 123.029, 245.303 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.480 - 2.100 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.20700 |
| Structure solution method | OTHER |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.637 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.500 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.130 | 0.500 |
| Number of reflections | 101798 | |
| <I/σ(I)> | 22 | 7.8 |
| Completeness [%] | 97.4 | 100 |
| Redundancy | 9.7 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | pH 9.00 |
