2J4Y
Crystal structure of a rhodopsin stabilizing mutant expressed in mammalian cells
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 |
| Unit cell lengths | 109.300, 109.300, 77.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.400 |
| R-factor | 0.2896 |
| Rwork | 0.290 |
| R-free | 0.32990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gzm |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.660 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.500 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.240 | 0.700 |
| Number of reflections | 13689 | |
| <I/σ(I)> | 5.42 | 1.79 |
| Completeness [%] | 95.9 | 84.8 |
| Redundancy | 3.03 | 2.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | VAPOUR DIFFUSION IN SITTING DROPS OF 10-15 MG/ML PROTEIN AND 0.2% C8E4,0.05%LDAO AGAINST 1.1-1.7M LITHIUM SULFATE, 0.1M HEPES PH 7.5 |
