2J4X

Streptococcus dysgalactiae-derived mitogen (SDM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	289
Detector technology	CCD
Collection date	2005-01-28
Detector	MARRESEARCH
Spacegroup name	P 32
Unit cell lengths	51.868, 51.868, 62.104
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 1.950
R-factor	0.205
Rwork	0.205
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1an8
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.050	0.420
Number of reflections	13696
<I/σ(I)>	21.2	2.5
Completeness [%]	96.3	90.8
Redundancy	7.4	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.2		18-20% PEG3350, 0.4M LINO3 PH 4.2-4.4